Fluorescence colocalization microscopy analysis can be improved by combining object-recognition with pixel-intensity-correlation.

ABSTRACT: The question whether two proteins interact with each other or whether a protein localizes to a certain region of the cell is often addressed with fluorescence microscopy and analysis of a potential colocalization of fluorescence markers. Since a mere visual estimation does not allow quantification of the degree of colocalization, different statistical methods of pixel-intensity correlation are commonly used to score it. We observed that these correlation coefficients are prone to false positive results and tend to show high values even for molecules that reside in different organelles. Our aim was to improve this type of analysis and we developed a novel method combining object-recognition based colocalization analysis with pixel-intensity correlation to calculate an object-corrected Pears

SUBMITTER: Moser B 

PROVIDER: S-EPMC5244660 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature