Vaccine candidates derived from a novel infectious cDNA clone of an American genotype dengue virus type 2.

ABSTRACT:

Background

A dengue virus type 2 (DEN-2 Tonga/74) isolated from a 1974 epidemic was characterized by mild illness and belongs to the American genotype of DEN-2 viruses. To prepare a vaccine candidate, a previously described 30 nucleotide deletion (Delta30) in the 3' untranslated region of DEN-4 has been engineered into the DEN-2 isolate.

Methods

A full-length cDNA clone was generated from the DEN-2 virus and used to produce recombinant DEN-2 (rDEN-2) and rDEN2Delta30. Viruses were evaluated for replication in SCID mice transplanted with human hepatoma cells (SCID-HuH-7 mice), in mosquitoes, and in rhesus monkeys. Neutralizing antibody induction and protective efficacy were also assessed in rhesus monkeys.

Results

The rDEN2Delta30 virus was ten-fold reduced in replicat

SUBMITTER: Blaney JE 

PROVIDER: S-EPMC524489 | biostudies-literature | 2004 Oct

REPOSITORIES: biostudies-literature