Human SRMAtlas: A Resource of Targeted Assays to Quantify the Complete Human Proteome.

ABSTRACT: The ability to reliably and reproducibly measure any protein of the human proteome in any tissue or cell type would be transformative for understanding systems-level properties as well as specific pathways in physiology and disease. Here, we describe the generation and verification of a compendium of highly specific assays that enable quantification of 99.7% of the 20,277 annotated human proteins by the widely accessible, sensitive, and robust targeted mass spectrometric method selected reaction monitoring, SRM. This human SRMAtlas provides definitive coordinates that conclusively identify the respective peptide in biological samples. We report data on 166,174 proteotypic peptides providing multiple, independent assays to quantify any human protein and numerous spliced variants, non-synony

SUBMITTER: Kusebauch U 

PROVIDER: S-EPMC5245710 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature