Use of (113)Cd NMR to probe the native metal binding sites in metalloproteins: an overview.

ABSTRACT: Our laboratories have actively published in this area for several years and the objective of this chapter is to present as comprehensive an overview as possible. Following a brief review of the basic principles associated with (113)Cd NMR methods, we will present the results from a thorough literature search for (113)Cd chemical shifts from metalloproteins. The updated (113)Cd chemical shift figure in this chapter will further illustrate the excellent correlation of the (113)Cd chemical shift with the nature of the coordinating ligands (N, O, S) and coordination number/geometry, reaffirming how this method can be used not only to identify the nature of the protein ligands in uncharacterized cases but also the dynamics at the metal binding site. Specific examples will be drawn from studies

SUBMITTER: Armitage IM 

PROVIDER: S-EPMC5245840 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature