Computational modeling reveals how interplay between components of a GTPase-cycle module regulates signal transduction.

ABSTRACT: Heterotrimeric G protein signaling is regulated by signaling modules composed of heterotrimeric G proteins, active G protein-coupled receptors (Rs), which activate G proteins, and GTPase-activating proteins (GAPs), which deactivate G proteins. We term these modules GTPase-cycle modules. The local concentrations of these proteins are spatially regulated between plasma membrane microdomains and between the plasma membrane and cytosol, but no data or models are available that quantitatively explain the effect of such regulation on signaling. We present a computational model of the GTPase-cycle module that predicts that the interplay of local G protein, R, and GAP concentrations gives rise to 16 distinct signaling regimes and numerous intermediate signaling phenomena. The regimes suggest alter

SUBMITTER: Bornheimer SJ 

PROVIDER: S-EPMC524695 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature