Targeted DNA sequencing and in situ mutation analysis using mobile phone microscopy.

ABSTRACT: Molecular diagnostics is typically outsourced to well-equipped centralized laboratories, often far from the patient. We developed molecular assays and portable optical imaging designs that permit on-site diagnostics with a cost-effective mobile-phone-based multimodal microscope. We demonstrate that targeted next-generation DNA sequencing reactions and in situ point mutation detection assays in preserved tumour samples can be imaged and analysed using mobile phone microscopy, achieving a new milestone for tele-medicine technologies.

SUBMITTER: Kuhnemund M 

PROVIDER: S-EPMC5247573 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature