Targeted DNA sequencing and in situ mutation analysis using mobile phone microscopy.
Ontology highlight
ABSTRACT:
SUBMITTER: Kuhnemund M
PROVIDER: S-EPMC5247573 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Publications
Targeted DNA sequencing and in situ mutation analysis using mobile phone microscopy.
Kühnemund Malte M Wei Qingshan Q Darai Evangelia E Wang Yingjie Y Hernández-Neuta Iván I Yang Zhao Z Tseng Derek D Ahlford Annika A Mathot Lucy L Sjöblom Tobias T Ozcan Aydogan A Nilsson Mats M
Nature communications 20170117
Molecular diagnostics is typically outsourced to well-equipped centralized laboratories, often far from the patient. We developed molecular assays and portable optical imaging designs that permit on-site diagnostics with a cost-effective mobile-phone-based multimodal microscope. We demonstrate that targeted next-generation DNA sequencing reactions and in situ point mutation detection assays in preserved tumour samples can be imaged and analysed using mobile phone microscopy, achieving a new mile ...[more]
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