Comparative genomics and transcriptome analysis of Aspergillus niger and metabolic engineering for citrate production.

ABSTRACT: Despite a long and successful history of citrate production in Aspergillus niger, the molecular mechanism of citrate accumulation is only partially understood. In this study, we used comparative genomics and transcriptome analysis of citrate-producing strains-namely, A. niger H915-1 (citrate titer: 157 g L^-1), A1 (117 g L^-1), and L2 (76 g L^-1)-to gain a genome-wide view of the mechanism of citrate accumulation. Compared with A. niger A1 and L2, A. niger H915-1 contained 92 mutated genes, including a succinate-semialdehyde dehydrogenase in the γ-aminobutyric acid shunt pathway and an aconitase family protein involved in citrate synthesis. Furthermore, transcriptome analysis of A. niger H915-1 revealed that the transcription levels of 479 genes changed betwee

SUBMITTER: Yin X 

PROVIDER: S-EPMC5247736 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature