Shigatoxin encoding Bacteriophage ϕ24_B modulates bacterial metabolism to raise antimicrobial tolerance.

ABSTRACT: How temperate bacteriophages play a role in microbial infection and disease progression is not fully understood. They do this in part by carrying genes that promote positive evolutionary selection for the lysogen. Using Biolog phenotype microarrays and comparative metabolite profiling we demonstrate the impact of the well-characterised Shiga toxin-prophage ϕ24_B on its Escherichia coli host MC1061. As a lysogen, the prophage alters the bacterial physiology by increasing the rates of respiration and cell proliferation. This is the first reported study detailing phage-mediated control of the E. coli biotin and fatty acid synthesis that is rate limiting to cell growth. Through ϕ24_B conversion the lysogen also gains increased antimicrobial tolerance to chloroxylenol and 8-

SUBMITTER: Holt GS 

PROVIDER: S-EPMC5247750 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature