Comparison of different sample preparation methods for platinum determination in cultured cells by graphite furnace atomic absorption spectrometry.

ABSTRACT:

Background

Platinum-based agents are widely used in chemotherapy against solid tumors and insufficient intracellular drug accumulation is one of the leading causes of platinum resistance which is associated with poor survival of tumor patients. Thus, the detection of intracellular platinum is pivotal for studies aiming to overcome platinum resistance. In the present study, we aimed to establish a reliable graphite furnace atomic absorption spectrometry (GFAAS)-based assay to quantify the intracellular platinum content for cultured cells.

Methods

Several most commonly applied cell preparation methods, including 0.2% HNO₃, 0.2% Triton X-100, concentrated nitric acid, RIPA combined with concentrated nitric acid and hydroxide, followed by GFAAS for platinum detection

SUBMITTER: Xiao M 

PROVIDER: S-EPMC5248575 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature