Homology modeling and molecular dynamics provide structural insights into tospovirus nucleoprotein.

ABSTRACT:

Background

Tospovirus is a plant-infecting genus within the family Bunyaviridae, which also includes four animal-infecting genera: Hantavirus, Nairovirus, Phlebovirus and Orthobunyavirus. Compared to these members, the structures of Tospovirus proteins still are poorly understood. Despite multiple studies have attempted to identify candidate N protein regions involved in RNA binding and protein multimerization for tospovirus using yeast two-hybrid systems (Y2HS) and site-directed mutagenesis, the tospovirus ribonucleocapsids (RNPs) remains largely uncharacterized at the molecular level and the lack of structural information prevents detailed insight into these interactions.

Results

Here we used the nucleoprotein structure of LACV (La Crosse virus-Orthobunyavirus) and molecul

SUBMITTER: Lima RN 

PROVIDER: S-EPMC5249003 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature