Long-term live-cell imaging reveals new roles for Salmonella effector proteins SseG and SteA.

ABSTRACT: Salmonella Typhimurium is an intracellular bacterial pathogen that infects both epithelial cells and macrophages. Salmonella effector proteins, which are translocated into the host cell and manipulate host cell components, control the ability to replicate and/or survive in host cells. Due to the complexity and heterogeneity of Salmonella infections, there is growing recognition of the need for single-cell and live-cell imaging approaches to identify and characterize the diversity of cellular phenotypes and how they evolve over time. Here, we establish a pipeline for long-term (17 h) live-cell imaging of infected cells and subsequent image analysis methods. We apply this pipeline to track bacterial replication within the Salmonella-containing vacuole in epithelial cells, quantify vacuolar r

SUBMITTER: McQuate SE 

PROVIDER: S-EPMC5249251 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature