Periostin regulates fibrocyte function to promote myofibroblast differentiation and lung fibrosis.

ABSTRACT: Fibrocytes are circulating mesenchymal precursors (CD45+, col 1+) recruited to fibrotic areas. Fibrocytes secrete profibrotic mediators including periostin; a matricellular protein that regulates cellular interactions with extracellular matrix (ECM) components. In bleomycin-induced fibrosis, periostin deficiency in structural or hematopoietic cells limits development of pulmonary fibrosis. To determine if hematopoietic-derived fibrocytes might secrete soluble factors to activate structural myofibroblast differentiation, wild-type (WT) fibroblasts were treated with conditioned medium from fibrocytes isolated from bleomycin-treated WT or periostin^-/- mice. After 24 h we saw less α-smooth muscle actin expression in cells treated with conditioned medium from periostin^-/-

SUBMITTER: Ashley SL 

PROVIDER: S-EPMC5250615 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature