Unknown

Dataset Information

0

Combined phospholipid biomarker-16S rRNA gene denaturing gradient gel electrophoresis analysis of bacterial diversity and physiological status in an intertidal microbial mat.

ABSTRACT: A combined lipid biomarker-16S rRNA gene denaturing gradient gel electrophoresis analysis was used to monitor changes in the physiological status, biomass, and microbial composition of a microbial mat. In the morning hours, an increase in the biomass of layers containing a high density of phototrophs and a decrease in the growth rate in the deep layers were observed. The combined approach also revealed differences in major groups of microorganisms, including green nonsulfur, gram-positive, and heterotrophic bacteria.

SUBMITTER: Villanueva L 

PROVIDER: S-EPMC525114 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Combined phospholipid biomarker-16S rRNA gene denaturing gradient gel electrophoresis analysis of bacterial diversity and physiological status in an intertidal microbial mat.

Villanueva Laura L   Navarrete Antoni A   Urmeneta Jordi J   White David C DC   Guerrero Ricardo R  

Applied and environmental microbiology 20041101 11

A combined lipid biomarker-16S rRNA gene denaturing gradient gel electrophoresis analysis was used to monitor changes in the physiological status, biomass, and microbial composition of a microbial mat. In the morning hours, an increase in the biomass of layers containing a high density of phototrophs and a decrease in the growth rate in the deep layers were observed. The combined approach also revealed differences in major groups of microorganisms, including green nonsulfur, gram-positive, and h  ...[more]

Similar Datasets

Unknown Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community.
| S-EPMC167804 | biostudies-other
Unknown Seasonal distributions of dominant 16S rRNA-defined populations in a hot spring microbial mat examined by denaturing gradient gel electrophoresis.
| S-EPMC168431 | biostudies-other
Unknown Analysis of bacterial communities in the rhizosphere of chrysanthemum via denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA as well as DNA fragments coding for 16S rRNA.
| S-EPMC92540 | biostudies-literature
Unknown High-resolution differentiation of Cyanobacteria by using rRNA-internal transcribed spacer denaturing gradient gel electrophoresis.
| S-EPMC262283 | biostudies-literature
Unknown Evaluations of different hypervariable regions of archaeal 16S rRNA genes in profiling of methanogens by Archaea-specific PCR and denaturing gradient gel electrophoresis.
| S-EPMC2227698 | biostudies-literature
Unknown Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis.
| S-EPMC3019698 | biostudies-literature
Unknown Denaturing gradient gel electrophoresis analysis of the 16S rRNA gene V1 region to monitor dynamic changes in the bacterial population during fermentation of Italian sausages.
| S-EPMC93279 | biostudies-literature
Unknown Identification of bacteria in a biodegraded wall painting by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA.
| S-EPMC167983 | biostudies-other
Unknown Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation.
| S-EPMC1169018 | biostudies-literature
Unknown Detection of rifampin resistance in Mycobacterium tuberculosis by double gradient-denaturing gradient gel electrophoresis.
| S-EPMC89519 | biostudies-literature