DNA Macroarray profiling of Lactococcus lactis subsp. lactis IL1403 gene expression during environmental stresses.

ABSTRACT: This report describes the use of an oligonucleotide macroarray to profile the expression of 375 genes in Lactococcus lactis subsp. lactis IL1403 during heat, acid, and osmotic stress. A set of known stress-associated genes in IL1403 was used as the internal control on the array. Every stress response was accurately detected using the macroarray, compared to data from previous reports. As a group, the expression patterns of the investigated metabolic genes were significantly altered by heat, acid, and osmotic stresses. Specifically, 13 to 18% of the investigated genes were differentially expressed in each of the environmental stress treatments. Interestingly, the methionine biosynthesis pathway genes (metA-metB1 and metB2-cysK) were induced during heat shock, but methionine utilization gene

SUBMITTER: Xie Y 

PROVIDER: S-EPMC525116 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature