Microscopic and Molecular Detection of Cryptosporidium andersoni and Cryptosporidium xiaoi in Wastewater Samples of Tehran Province, Iran.

ABSTRACT:

Background

As a waterborne pathogen, Cryptosporidium is one of the most common causes of gastroenteritis in human and hoofed livestock animals. This study aimed to investigate the distribution of Cryptosporidium spp. in human and livestock wastewaters in Iran, by the 18S rRNA sequence analysis.

Methods

A total of 54 raw wastewater samples collected from three urban treatment plants and two slaughterhouses during 2014-2015 in Tehran, Iran. The presence of the Cryptosporidium oocysts was assessed by immunofluorescence with monoclonal antibodies. To characterize the oocysts at the molecular level, the 18S rRNA gene of Cryptosporidium was PCR amplified and sequenced.

Results

Of the 54 wastewater samples examined, 34 (62.9%) were positive for

SUBMITTER: Hatam-Nahavandi K 

PROVIDER: S-EPMC5251178 | biostudies-literature | 2016 Oct-Dec

REPOSITORIES: biostudies-literature