Sorting nexin-4 regulates β-amyloid production by modulating β-site-activating cleavage enzyme-1.

ABSTRACT:

Background

Amyloid precursor protein (APP) is cleaved by β-site amyloid precursor protein-cleaving enzyme 1 (BACE1) to produce β-amyloid (Aβ), a critical pathogenic peptide in Alzheimer's disease (AD). Aβ generation can be affected by the intracellular trafficking of APP or its related secretases, which is thus important to understanding its pathological alterations. Although sorting nexin (SNX) family proteins regulate this trafficking, the relevance and role of sorting nexin-4 (SNX4) regarding AD has not been studied yet.

Methods

In this study, human brain tissue and APP/PS1 mouse brain tissue were used to check the disease relevance of SNX4. To investigate the role of SNX4 in AD pathogenesis, several experiments were done, such as coimmunoprecipitation, Western blotting,

SUBMITTER: Kim NY 

PROVIDER: S-EPMC5251330 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature