Display of biologically functional insecticidal toxin on the surface of lambda phage.

ABSTRACT: The successful use of Bacillus thuringiensis insecticidal toxins to control agricultural pests could be undermined by the evolution of insect resistance. Under selection pressure in the laboratory, a number of insects have gained resistance to the toxins, and several cases of resistance in the diamondback moth have been reported from the field. The use of protein engineering to develop novel toxins active against resistant insects could offer a solution to this problem. The display of proteins on the surface of phages has been shown to be a powerful technology to search for proteins with new characteristics from combinatorial libraries. However, this potential of phage display to develop Cry toxins with new binding properties and new target specificities has hitherto not been realized beca

SUBMITTER: Vilchez S 

PROVIDER: S-EPMC525175 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature