Promoter sequences necessary for high-level expression of the plasmid-associated ampC beta-lactamase gene blaMIR-1.

ABSTRACT: Little is known about mechanisms involved in high-level expression of plasmid-associated ampC genes. The sequence for bla(MIR-1) has been elucidated, and the gene is not inducible. Although the sequence for the promoter (prA) that drives expression of Enterobacter cloacae chromosomal ampC is present upstream of bla(MIR-1), high-level expression from bla(MIR-1) is directed from a hybrid promoter (prB) located further upstream of prA. The purpose of this study was to determine the influence of each promoter on bla(MIR-1) expression and beta-lactam resistance. RNA expression by deletion clones with both promoters was measured and compared to that by clones in which -35 and/or -10 elements of prA and/or prB were altered. Primer extension revealed two start sites for bla(MIR-1) transcription. E

SUBMITTER: Reisbig MD 

PROVIDER: S-EPMC525406 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature