Inducible gene trapping with drug-selectable markers and Cre/loxP to identify developmentally regulated genes.

ABSTRACT: Gene trapping in mouse embryonic stem cells is an important genetic approach that allows simultaneous mutation of genes and generation of corresponding mutant mice. We designed a selection scheme with drug selection markers and Cre/loxP technology which allows screening of gene trap events that responded to a signaling molecule in a 96-well format. Nine hundred twenty gene trap clones were assayed, and 258 were classified as gene traps induced by in vitro differentiation. Sixty-five of the in vitro differentiation-inducible gene traps were also responsive to retinoic acid treatment. In vivo analysis revealed that 85% of the retinoic acid-inducible gene traps trapped developmentally regulated genes, consistent with the observation that genes induced by retinoic acid treatment are likely to

SUBMITTER: Chen YT 

PROVIDER: S-EPMC525470 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature