Genome and transcriptome analysis of surfactin biosynthesis in Bacillus amyloliquefaciens MT45.

ABSTRACT: Natural Bacillus isolates generate limited amounts of surfactin (<10% of their biomass), which functions as an antibiotic or signalling molecule in inter-/intra-specific interactions. However, overproduction of surfactin in Bacillus amyloliquefaciens MT45 was observed at a titre of 2.93 g/l, which is equivalent to half of the maximum biomass. To systemically unravel this efficient biosynthetic process, the genome and transcriptome of this bacterium were compared with those of B. amyloliquefaciens type strain DSM7^T. MT45 possesses a smaller genome while containing more unique transporters and resistance-associated genes. Comparative transcriptome analysis revealed notable enrichment of the surfactin synthesis pathway in MT45, including central carbon metabolism and fatty acid bio

SUBMITTER: Zhi Y 

PROVIDER: S-EPMC5256033 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature