Structure-based modelling of hemocyanin allergenicity in squid and its response to high hydrostatic pressure.

ABSTRACT: The secondary, tertiary, and quaternary structures of squid hemocyanin (Hc) were characterised, and the relationship between Hc structure and allergenicity responses to high hydrostatic pressure (HHP) was modelled. The Hc allergenicity varied with its protein structure. Electrophoresis analysis revealed that HHP treatment significantly decreased the band intensity of Hc when increasing pressure from 200 and 400 MPa to 600 MPa. The protein structure analysis of squid Hc showed that while HHP treatment decreased the α-helix content, free sulfhydryl content, and R_g, it increased the random coil content, surface hydrophobicity index (Ho), Guinier aggregation number (〈N_agg〉_G) and average aggregation number (〈N_agg〉_Q). The α-helix and random

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC5256278 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature