An active murine transposon family pair: retrotransposition of "master" MusD copies and ETn trans-mobilization.

ABSTRACT: The ETn (Early Transposon) elements are among the most active murine mobile sequences, being responsible for a series of mutations by insertion in vivo. Yet they are noncoding, and it had long been suspected that ETn are mobilized in trans by coding-competent elements, most probably from the closely related MusD family of LTR-retrotransposons. A genome-wide in silico search for coding-competent MusD elements identified a total of nine such copies, which we cloned and marked to test their transpositional activity, using an ex vivo assay in heterologous cells. Three copies were found to be autonomous for transposition, with each gag, pro, and pol MusD gene absolutely required for mobility. These active MusD copies specifically trigger retrotransposition of marked ETn elements with high effic

SUBMITTER: Ribet D 

PROVIDER: S-EPMC525684 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature