Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling.

ABSTRACT: The comprehensive understanding of cellular signaling pathways remains a challenge due to multiple layers of regulation that may become evident only when the pathway is probed at different levels or critical nodes are eliminated. To discover regulatory mechanisms in canonical WNT signaling, we conducted a systematic forward genetic analysis through reporter-based screens in haploid human cells. Comparison of screens for negative, attenuating and positive regulators of WNT signaling, mediators of R-spondin-dependent signaling and suppressors of constitutive signaling induced by loss of the tumor suppressor adenomatous polyposis coli or casein kinase 1α uncovered new regulatory features at most levels of the pathway. These include a requirement for the transcription factor AP-4, a role for t

SUBMITTER: Lebensohn AM 

PROVIDER: S-EPMC5257257 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature