Imaging-cytometry revealed spatial heterogeneities of marker expression in undifferentiated human pluripotent stem cells.

ABSTRACT: Human pluripotent stem cells (hPSCs) provide a good model system for studying human development and are expected as a source for both cell-based medical and pharmaceutical research application. However, stable maintenance of undifferentiated hPSCs is yet challenging, and thus routine characterization is required. Flow-cytometry is one of the popular quantitative characterization tools for hPSCs, but it has drawback of spatial information loss of the cells in the culture. Here, we have applied a two-dimensional imaging cytometry that examines undifferentiated state of hPSCs to analyze localization and morphological information of immunopositive cells in the culture. The whole images of cells in a culture vessel were acquired and analyzed by an image analyzer, IN Cell Analyzer 2000, and dete

SUBMITTER: Suga M 

PROVIDER: S-EPMC5258813 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature