SOX9 protein is stabilized by TGF-β and regulates PAPSS2 mRNA expression in chondrocytes.

ABSTRACT:

Objective

We previously identified 3'-phosphoadenosine 5'-phosphosulfate synthase 2 (PAPSS2) as a transcriptional target of transforming growth factor β (TGF-β) in chondrocytes. PAPSS2 is required for proper sulfation of proteoglycans in cartilage. Defective sulfation in the matrix results in alterations in mechanical properties of the cartilage that would be expected to result in degeneration. The objective of this study was to identify factors that regulate PAPSS2 expression and compare to a known TGF-β responsive gene, proteoglycan 4/lubricin (PRG4). In this study, TGF-β-mediated regulation of SOX9 was characterized, and the involvement of SOX9 in regulation of PAPSS2 mRNA was investigated.

Design

Primary bovine articular chondrocytes grown in micromass culture and ATDC5

SUBMITTER: Chavez RD 

PROVIDER: S-EPMC5258840 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature