Genome-wide analysis of mRNAs associated with mouse peroxisomes.

ABSTRACT:

Background

RNA is often targeted to be localized to the specific subcellular compartments. Specific localization of mRNA is believed to be an important mechanism for targeting their protein products to the locations, where their function is required.

Results

In this study we performed the genome wide transcriptome analysis of peroxisome preparations from the mouse liver using microarrays. We demonstrate that RNA is absent inside peroxisomes, however it is associated at their exterior via the noncovalent contacts with the membrane proteins. We detect enrichment of specific sets of transcripts in two preparations of peroxisomes, purified with different degrees of stringency. Importantly, among these were mRNAs encoding bona fide peroxisomal proteins, such as peroxins and perox

SUBMITTER: Yarmishyn AA 

PROVIDER: S-EPMC5259856 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature