Integrated analysis of hematopoietic differentiation outcomes and molecular characterization reveals unbiased differentiation capacity and minor transcriptional memory in HPC/HSC-iPSCs.

ABSTRACT:

Background

Transcription factor-mediated reprogramming can reset the epigenetics of somatic cells into a pluripotency compatible state. Recent studies show that induced pluripotent stem cells (iPSCs) always inherit starting cell-specific characteristics, called epigenetic memory, which may be advantageous, as directed differentiation into specific cell types is still challenging; however, it also may be unpredictable when uncontrollable differentiation occurs. In consideration of biosafety in disease modeling and personalized medicine, the availability of high-quality iPSCs which lack a biased differentiation capacity and somatic memory could be indispensable.

Methods

Herein, we evaluate the hematopoietic differentiation capacity and somatic memory state of hematopoietic pro

SUBMITTER: Gao S 

PROVIDER: S-EPMC5259886 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature