Single-cell epigenomic variability reveals functional cancer heterogeneity.

ABSTRACT:

Background

Cell-to-cell heterogeneity is a major driver of cancer evolution, progression, and emergence of drug resistance. Epigenomic variation at the single-cell level can rapidly create cancer heterogeneity but is difficult to detect and assess functionally.

Results

We develop a strategy to bridge the gap between measurement and function in single-cell epigenomics. Using single-cell chromatin accessibility and RNA-seq data in K562 leukemic cells, we identify the cell surface marker CD24 as co-varying with chromatin accessibility changes linked to GATA transcription factors in single cells. Fluorescence-activated cell sorting of CD24 high versus low cells prospectively isolated GATA1 and GATA2 high versus low cells. GATA high versus low cells express differential gene regu

SUBMITTER: Litzenburger UM 

PROVIDER: S-EPMC5259890 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature