Multi-CAR: a tool of contig scaffolding using multiple references.

ABSTRACT:

Background

A draft genome assembled by current next-generation sequencing techniques from short reads is just a collection of contigs, whose relative positions and orientations along the genome being sequenced are unknown. To further obtain its complete sequence, a contig scaffolding process is usually applied to order and orient the contigs in the draft genome. Although several single reference-based scaffolding tools have been proposed, they may produce erroneous scaffolds if there are rearrangements between the target and reference genomes or their phylogenetic relationship is distant. This may suggest that a single reference genome may not be sufficient to produce correct scaffolds of a draft genome.

Results

In this study, we design a simple heuristic method to further r

SUBMITTER: Chen KT 

PROVIDER: S-EPMC5260120 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature