Human fetal intestinal epithelial cells metabolize and incorporate branched chain fatty acids in a structure specific manner.

ABSTRACT:

Background

Branched chain fatty acids (BCFA) are constituents of gastrointestinal (GI) tract in healthy newborn human infants, reduce the incidence of necrotizing enterocolitis (NEC) in a neonatal rat model, and are incorporated into small intestine cellular lipids in vivo. We hypothesize that BCFA are taken up, metabolized and incorporated into human fetal cells in vitro.

Methods

Human H4 cells, a fetal non-transformed primary small intestine cell line, were incubated with albumin-bound non-esterified anteiso-17:0, iso-16:0, iso-18:0 and/or iso-20:0, and FA profiles in lipid fractions were analyzed.

Results

All BCFA were readily incorporated as major constituents of cellular lipids. Anteiso-17:0 was preferentially taken up, and was most effective among BCFA tested in

SUBMITTER: Liu L 

PROVIDER: S-EPMC5260611 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature