Epigenetic programming of Dnmt3a mediated by AP2α is required for granting preadipocyte the ability to differentiate.

ABSTRACT: Adipogenesis has an important role in regulating energy homeostasis in mammals. 3T3-L1 preadipocytes have been widely used as an in vitro model for analyzing the molecular mechanism of adipogenesis. Previous reports indicated that the stage of contact inhibition (CI), through which the proliferating cells exit from the cell cycle, was required for granting preadipocyte the ability to differentiate. While this kind of the granting mechanism remains elusive. In the present study, we showed that DNA (cytosine-5) methyltransferase 3a (Dnmt3a) was upregulated at both the mRNA and protein level during the CI stage, and resulted in increasing promoter methylation of adipogenic genes. We further identified that the expression of Activator protein 2α (AP2α), a member of the transcription factor act

SUBMITTER: Guo W 

PROVIDER: S-EPMC5261006 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature