Sensitivity of hematopoietic stem cells to mitochondrial dysfunction by SdhD gene deletion.

ABSTRACT: It is established that hematopoietic stem cells (HSC) in the hypoxic bone marrow have adapted their metabolism to oxygen-limiting conditions. This adaptation includes suppression of mitochondrial activity, induction of anerobic glycolysis, and activation of hypoxia-inducible transcription factor 1α (Hif1α)-dependent gene expression. During progression of hematopoiesis, a metabolic switch towards mitochondrial oxidative phosphorylation is observed, making this organelle essential for determining cell fate choice in bone marrow. However, given that HSC metabolism is essentially oxygen-independent, it is still unclear whether functional mitochondria are absolutely required for their survival. To assess the actual dependency of these undifferentiated cells on mitochondrial function, we have pe

SUBMITTER: Bejarano-Garcia JA 

PROVIDER: S-EPMC5261010 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature