Cloning of a novel inhibin alpha cDNA from rhesus monkey testis.

ABSTRACT:

Background

Inhibins are dimeric gonadal protein hormones that negatively regulate pituitary FSH synthesis and secretion. Inhibin B is produced by testicular Sertoli cells and is the primary circulating form of inhibin in most adult male mammals. Inhibin B is comprised of the inhibin alpha subunit disulfide-linked to the inhibin/activin betaB subunit. Here we describe the cloning of the cDNAs encoding these subunits from adult rhesus monkey testis RNA.

Methods

The subunit cDNAs were cloned by a combination of reverse transcriptase polymerase chain reaction (RT-PCR) and 5' rapid amplification of cDNA ends (RACE) RT-PCR from adult rhesus monkey testis RNA.

Results

Both the inhibin alpha and betaB subunit nucleotide and predicted protein sequences are highly conserved wit

SUBMITTER: Bernard DJ 

PROVIDER: S-EPMC526212 | biostudies-literature | 2004 Oct

REPOSITORIES: biostudies-literature