Human Muse Cells Reconstruct Neuronal Circuitry in Subacute Lacunar Stroke Model.

ABSTRACT:

Background and purpose

Multilineage-differentiating stress-enduring (muse) cells are endogenous nontumorigenic stem cells with pluripotency harvestable as pluripotent marker SSEA-3⁺ cells from the bone marrow from cultured bone marrow-mesenchymal stem cells. After transplantation into neurological disease models, muse cells exert repair effects, but the exact mechanism remains inconclusive.

Methods

We conducted mechanism-based experiments by transplanting serum/xeno-free cultured-human bone marrow-muse cells into the perilesion brain at 2 weeks after lacunar infarction in immunodeficient mice.

Results

Approximately 28% of initially transplanted muse cells remained in the host brain at 8 weeks, spontaneously differentiated into cells expressing NeuN (≈62%),

SUBMITTER: Uchida H 

PROVIDER: S-EPMC5262965 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature