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Dynamin-2 Stabilizes the HIV-1 Fusion Pore with a Low Oligomeric State.

ABSTRACT: One of the key research areas surrounding HIV-1 concerns the regulation of the fusion event that occurs between the virus particle and the host cell during entry. Even if it is universally accepted that the large GTPase dynamin-2 is important during HIV-1 entry, its exact role during the first steps of HIV-1 infection is not well characterized. Here, we have utilized a multidisciplinary approach to study the DNM2 role during fusion of HIV-1 in primary resting CD4 T and TZM-bl cells. We have combined advanced light microscopy and functional cell-based assays to experimentally assess the role of dynamin-2 during these processes. Overall, our data suggest that dynamin-2, as a tetramer, might help to establish hemi-fusion and stabilizes the pore during HIV-1 fusion.

SUBMITTER: Jones DM

PROVIDER: S-EPMC5263234 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Dynamin-2 Stabilizes the HIV-1 Fusion Pore with a Low Oligomeric State.

Jones Daniel M DM Alvarez Luis A LA Nolan Rory R Ferriz Margarita M Sainz Urruela Raquel R Massana-Muñoz Xènia X Novak-Kotzer Hila H Dustin Michael L ML Padilla-Parra Sergi S

Cell reports 20170101 2

One of the key research areas surrounding HIV-1 concerns the regulation of the fusion event that occurs between the virus particle and the host cell during entry. Even if it is universally accepted that the large GTPase dynamin-2 is important during HIV-1 entry, its exact role during the first steps of HIV-1 infection is not well characterized. Here, we have utilized a multidisciplinary approach to study the DNM2 role during fusion of HIV-1 in primary resting CD4 T and TZM-bl cells. We have comb ...[more]

PMID: 28076788

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