The Matrix protein M1 from influenza C virus induces tubular membrane invaginations in an in vitro cell membrane model.

ABSTRACT: Matrix proteins from enveloped viruses play an important role in budding and stabilizing virus particles. In order to assess the role of the matrix protein M1 from influenza C virus (M1-C) in plasma membrane deformation, we have combined structural and in vitro reconstitution experiments with model membranes. We present the crystal structure of the N-terminal domain of M1-C and show by Small Angle X-Ray Scattering analysis that full-length M1-C folds into an elongated structure that associates laterally into ring-like or filamentous polymers. Using negatively charged giant unilamellar vesicles (GUVs), we demonstrate that M1-C full-length binds to and induces inward budding of membrane tubules with diameters that resemble the diameter of viruses. Membrane tubule formation requires the C-ter

SUBMITTER: Saletti D 

PROVIDER: S-EPMC5264427 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature