Mechanical Characterization of Microengineered Epithelial Cysts by Using Atomic Force Microscopy.

ABSTRACT: Most organs contain interconnected tubular tissues that are one-cell-thick, polarized epithelial monolayers enclosing a fluid-filled lumen. Such tissue organization plays crucial roles in developmental and normal physiology, and the proper functioning of these tissues depends on their regulation by complex biochemical perturbations and equally important, but poorly understood, mechanical perturbations. In this study, by combining micropatterning techniques and atomic force microscopy, we developed a simple in vitro experimental platform for characterizing the mechanical properties of the MDCK II cyst, the simplest model of lumen-enclosing epithelial monolayers. By using this platform, we estimated the elasticity of the cyst monolayer and showed that the presence of a luminal space influenc

SUBMITTER: Shen Y 

PROVIDER: S-EPMC5266256 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature