Influence of High pH and Cholesterol on Single Arginine-Containing Transmembrane Peptide Helices.

ABSTRACT: An essential component of mammalian cells, cholesterol exerts significant influence on the physical properties of the cell membrane and in turn its constituents, including membrane proteins. Although sparse, polar amino acid residues are highly conserved in membrane proteins and play pivotal roles in determining specific structural and functional properties. To improve our understanding of particular polar residues in the membrane environment, we have examined two specific "guest" Arg residues within a well-defined and deuterium-labeled "host" framework provided by the transmembrane helical peptide GWALP23 (acetyl-GGALWLALALALALALALWLAGA-amide). Solid-state ²H nuclear magnetic resonance (NMR) spectra from aligned bilayer membrane samples effectively report changes in the host he

SUBMITTER: Thibado JK 

PROVIDER: S-EPMC5266483 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature