Mass Cytometry Analytical Approaches Reveal Cytokine-Induced Changes in Natural Killer Cells.

ABSTRACT:

Background

Natural killer (NK) cells have antiviral and antitumor activity that could be harnessed for the treatment of infections and malignancies. To maintain cell viability and enhance antiviral and antitumor effects, NK cells are frequently treated with cytokines. Here they performed an extensive assessment of the effects of cytokines on the phenotype and function of human NK cells.

Methods

They used cytometry by time-of-flight (CyTOF) to evaluate NK cell repertoire changes after stimulation with interleukin (IL)-2, IL-15 or a combination of IL-12/IL-15/IL-18. To analyze the high dimensional CyTOF data, they used several statistical and visualization tools, including viSNE (Visualization of t-Distributed Stochastic Neighbor Embedding), Citrus (Cluster identification, cha

SUBMITTER: Vendrame E 

PROVIDER: S-EPMC5266600 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature