Identification of Key Residues in the NisK Sensor Region for Nisin Biosynthesis Regulation.

ABSTRACT: Histidine kinase (HK) NisK is well known to sense lantibiotic nisin for regulating the biosynthesis of nisin. NisK possesses two trans-membrane segments and a large extracellular region and nisin contains 34 amino acids with five lanthionine rings. Unlike most peptide sensing HK with multi trans-membrane segments, NisK is a representative of a group of rarely reported HK that sense peptide as ligand. To reveal how NisK senses nisin molecule to regulate nisin biosynthesis, we constructed a reporter Lactococcus lactis strain with nisRK constitutively expressed and a reporter gene lacZ expressed under the control of promoter P _nisA . We showed that the extracellular region of NisK was involved in recognizing nisin. Conserved residues in this group of HK

SUBMITTER: Ge X 

PROVIDER: S-EPMC5266694 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature