Clathrin in <i>Chara australis</i>: Molecular Analysis and Involvement in Charasome Degradation and Constitutive Endocytosis.

ABSTRACT: Charasomes are convoluted plasma membrane domains in characean green algae. They are known to form in response to light via secretion of trans-Golgi network (TGN) vesicles and local inhibition of endocytosis. Charasomes are involved in the acidification of their aqueous environment, thereby facilitating photosynthesis-dependent carbon uptake. Charasome formation is reversible to allow cells to adapt to different light conditions. Here, we show that darkness-induced degradation of charasomes involves the formation of coated pits and coated vesicles. The darkness-induced degradation of charasomes can be inhibited by 1-2 μM ikarugamycin (IKA), which is considered to be a specific inhibitor of clathrin-dependent endocytosis. At a much higher concentration (100 μM), IKA also significantl

SUBMITTER: Hoepflinger MC 

PROVIDER: S-EPMC5266738 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature