Identification of enzymes responsible for extracellular alginate depolymerization and alginate metabolism in Vibrio algivorus.

ABSTRACT: Alginate is a marine non-food-competing polysaccharide that has potential applications in biorefinery. Owing to its large size (molecular weight >300,000 Da), alginate cannot pass through the bacterial cell membrane. Therefore, bacteria that utilize alginate are presumed to have an enzyme that degrades extracellular alginate. Recently, Vibrio algivorus sp. SA2^T was identified as a novel alginate-decomposing and alginate-utilizing species. However, little is known about the mechanism of alginate degradation and metabolism in this species. To address this issue, we screened the V. algivorus genomic DNA library for genes encoding polysaccharide-decomposing enzymes using a novel double-layer plate screening method and identified alyB as a candidate. Most identified alginate-decompos

SUBMITTER: Doi H 

PROVIDER: S-EPMC5266763 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature