The golgin coiled-coil proteins capture different types of transport carriers via distinct N-terminal motifs.

ABSTRACT:

Background

The internal organization of cells depends on mechanisms to ensure that transport carriers, such as vesicles, fuse only with the correct destination organelle. Several types of proteins have been proposed to confer specificity to this process, and we have recently shown that a set of coiled-coil proteins on the Golgi, called golgins, are able to capture specific classes of carriers when relocated to an ectopic location.

Results

Mapping of six different golgins reveals that, in each case, a short 20-50 residue region is necessary and sufficient to capture specific carriers. In all six of GMAP-210, golgin-84, TMF, golgin-97, golgin-245, and GCC88, this region is located at the extreme N-terminus of the protein. The vesicle-capturing regions of GMAP-210, golgin-84, a

SUBMITTER: Wong M 

PROVIDER: S-EPMC5267433 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature