Non-radioactive TRF assay modifications to improve telomeric DNA detection efficiency in plants.

ABSTRACT: The length of telomeric DNA is often considered a cellular biomarker of aging and general health status. Several telomere length measuring assays have been developed, of which the most common is the Telomere Restriction Fragment (TRF) analysis, which typically involves the use of radioactively labeled oligonucleotide probes. While highly effective, this method potentially poses substantial health concerns and generates radioactive waste. Digoxigenin (DIG) alternatives to radioactive probes have been developed and used successfully in a number of assays. Here we optimize the DIG protocol to measure telomere length in the model plant Arabidopsis thaliana and present evidence that this approach can be used successfully to efficiently and accurately measure telomere length in plants. Sp

SUBMITTER: Nigmatullina LR 

PROVIDER: S-EPMC5268086 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature