Profiling Living Bacteria Informs Preparation of Fecal Microbiota Transplantations.

ABSTRACT: Fecal microbiota transplantation is a compelling treatment for recurrent Clostridium difficile infections, with potential applications against other diseases associated with changes in gut microbiota. But variability in fecal bacterial communities-believed to be the therapeutic agent-can complicate or undermine treatment efficacy. To understand the effects of transplant preparation methods on living fecal microbial communities, we applied a DNA-sequencing method (PMA-seq) that uses propidium monoazide (PMA) to differentiate between living and dead fecal microbes, and we created an analysis pipeline to identify individual bacteria that change in abundance between samples. We found that oxygen exposure degraded fecal bacterial communities, whereas freeze-thaw cycles and lag time between dono

SUBMITTER: Chu ND 

PROVIDER: S-EPMC5268452 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature