Local Vascular Gene Therapy With Apolipoprotein A-I to Promote Regression of Atherosclerosis.

ABSTRACT:

Objective

Gene therapy, delivered directly to the blood vessel wall, could potentially prevent atherosclerotic lesion growth and promote atherosclerosis regression. Previously, we reported that a helper-dependent adenoviral (HDAd) vector expressing apolipoprotein A-I (apoA-I) in carotid endothelium of fat-fed rabbits reduced early (4 weeks) atherosclerotic lesion growth. Here, we tested whether the same HDAd-delivered to the existing carotid atherosclerotic lesions-could promote regression.

Approach and results

Rabbits (n=26) were fed a high-fat diet for 7 months, then treated with bilateral carotid gene transfer. One carotid was infused with an HDAd expressing apoA-I (HDAdApoAI) and the other with a control nonexpressing HDAd (HDAdNull). The side with HDAdApoAI was randomiz

SUBMITTER: Wacker BK 

PROVIDER: S-EPMC5269454 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature