P62/SQSTM1 enhances breast cancer stem-like properties by stabilizing MYC mRNA.

ABSTRACT: Aberrant p62 overexpression has been implicated in breast cancer development. Here, we found that p62 expression was elevated in breast cancer stem cells (BCSCs), including CD44⁺CD24^- fractions, mammospheres, ALDH1⁺ populations and side population cells. Indeed, short-hairpin RNA (shRNA)-mediated knockdown of p62 impaired breast cancer cells from self-renewing under anchorage-independent conditions, whereas ectopic overexpression of p62 enhanced the self-renewal ability of breast cancer cells in vitro. Genetic depletion of p62 robustly inhibited tumor-initiating frequencies, as well as growth rates of BCSC-derived tumor xenografts in immunodeficient mice. Consistently, immunohistochemical analysis of clinical breast tumor tissues showed that high p62 expres

SUBMITTER: Xu LZ 

PROVIDER: S-EPMC5269535 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature