Accessing the genomic information of unculturable oceanic picoeukaryotes by combining multiple single cells.

ABSTRACT: Pico-sized eukaryotes play key roles in the functioning of marine ecosystems, but we still have a limited knowledge on their ecology and evolution. The MAST-4 lineage is of particular interest, since it is widespread in surface oceans, presents ecotypic differentiation and has defied culturing efforts so far. Single cell genomics (SCG) are promising tools to retrieve genomic information from these uncultured organisms. However, SCG are based on whole genome amplification, which normally introduces amplification biases that limit the amount of genomic data retrieved from a single cell. Here, we increase the recovery of genomic information from two MAST-4 lineages by co-assembling short reads from multiple Single Amplified Genomes (SAGs) belonging to evolutionary closely related cells. We fo

SUBMITTER: Mangot JF 

PROVIDER: S-EPMC5269757 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature