Intraoperative qRT-PCR for detection of lymph node metastasis in head and neck cancer.

ABSTRACT:

Purpose

Sentinel node biopsy (SNB) has been shown to accurately stage the regional lymphatics in oral carcinoma. However, intraoperative pathology is only moderately sensitive and final pathology takes several days to complete. The purpose of this study was to develop a rapid, automated, and quantitative real-time PCR (qRT-PCR) assay that can match final pathology in an intraoperative time frame.

Experimental design

Four hundred forty-eight grossly tumor-negative lymph nodes were evaluated for expression of 3 markers [PVA (pemphigus vulgaris antigen), PTHrP (parathyroid hormone-related protein), and TACSTD1 (tumor-associated calcium signal transducer 1)]. Conformity of metastasis detection by qRT-PCR was determined using hematoxylin and eosin and immunohistochemistry stainin

SUBMITTER: Ferris RL 

PROVIDER: S-EPMC5270507 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature