Retinoic Acid Enhances the Differentiation of Adipose-Derived Stem Cells to Keratocytes In Vitro.

ABSTRACT:

Purpose

All-trans retinoic acid (RA) supplementation was investigated as a method of enhancing the differentiation of human adipose-derived stem cells (ASCs) to corneal keratocytes in vitro, in combination with a chemically defined serum-free medium.

Methods

Adipose-derived stem cells were cultured in monolayer and supplemented with 0.1, 1, or 10 μM RA for 14 days. The effects of RA on cell proliferation, migration, and extracellular matrix (ECM) accumulation were evaluated. In addition, the expression of phenotypic keratocyte markers was examined by reverse transcription polymerase chain reaction (PCR), immunocytochemistry, and Western blotting.

Results

Adipose-derived stem cells cultured with RA showed improved cell proliferation and ECM production. In addition, RA

SUBMITTER: Lynch AP 

PROVIDER: S-EPMC5270625 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature